CD35 (CR1) Mouse Monoclonal Antibody [Clone ID: E11]

SKU
SM1097P
CD35 (CR1) mouse monoclonal antibody, clone E11, Purified
  $645.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name E11
Application FC, IHC, WB
Recommended Dilution Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 106 cells or 100 µl whole blood.
Immunohistochemistry on Frozen Section: 1-10 µg/ml.
Immunohistochemistry on Paraffin Section: 1-10 µg/ml.
Requires antigen retrieval using heat treatment prior to staining of Paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Recommended Positive Control: Human Tonsil.
Western Blotting (Ref.3).
Reactivity Baboon, Human, Monkey
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human acute monocytic leukaemia cells.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity This antibody is specific for CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively.
Buffer PBS
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Gene Name complement component 3b/4b receptor 1 (Knops blood group)
Database Link
Background CD35 is a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement.
Synonyms Complement receptor type 1, CR1, C3b/C4b receptor
Reference Data
Protein Categories CD Markers, Membrane Proteins
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