Msr1 Rat Monoclonal Antibody [Clone ID: 2F8]

CAT#: SM029LE

Msr1 rat monoclonal antibody, clone 2F8, Low Endotoxin

Product Images

Staining of Mouse peritoneal Macrophages with Rat Anti Mouse CD204-LOW ENDOTOXIN.

Specifications

Product Data

• Clone Name: 2F8
• Applications: ELISA, FC, FN, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western blot: Recognises CD204 in J774 cells under Non-Reduced Conditions only.
  Functional assays.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Flow cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: RAW264 cell line.
• Specificity: This antibody recognises the Murine Scavenger Receptor Class A (SRA), type I and II, also known as CD204.
• Formulation: PBS, pH 7.4 without preservatives
  State: Low Endotoxin
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: macrophage scavenger receptor 1
• Database Link:
  Entrez Gene 20288 Mouse
  UniProt ID P30204
• Background: CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion.
• Synonyms: MSR1, SCARA1
• Note: Endotoxin Level: less than 0.01 EU/µg.
  Recent research shows that clone 2F8 recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.