Msr1 Rat Monoclonal Antibody [Clone ID: 2F8]

SKU SM029LE

Msr1 rat monoclonal antibody, clone 2F8, Low Endotoxin

Product Data

• Clone Name: 2F8
• Application: ELISA, FC, FN, IHC, IP, WB
• Recommended Dilution: ELISA.; Western blot: Recognises CD204 in J774 cells under Non-Reduced Conditions only.; Functional assays.; Immunoprecipitation.; Immunohistochemistry on Frozen Sections.; Flow cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
• Reactivity: Mouse
• Antibody Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: RAW264 cell line.
• Specificity: This antibody recognises the Murine Scavenger Receptor Class A (SRA), type I and II, also known as CD204.
• Buffer: PBS, pH 7.4 without preservatives; State: Low Endotoxin; State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: macrophage scavenger receptor 1
• Database Link:
  P30204
• Background: CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion.
• Synonyms: MSR1, SCARA1
• Note: Endotoxin Level: less than 0.01 EU/µg.; Recent research shows that clone 2F8 recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.

Reference Data