CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]
Specifications
| Product Data | |
| Clone Name | M1/70.15 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. |
| Reactivity | Human, Mouse, Rabbit |
| Antibody Host | Rat |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | T cell enriched splenocytes from B10 mice. Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line. |
| Specificity | This antibody recognises the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1). Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose. Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. |
| Buffer | PBS, pH 7.4 containing 0.09% Sodium Azide, 1% BSA, 5% Sucrose Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE) |
| Reconstitution Method | Restore with distilled water. |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | PE |
| Storage | Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. |
| Synonyms | ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1 |
| Reference Data | |
| Protein Categories | CD Markers, Intracellular Proteins, Membrane Proteins |
Reviews
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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