GFP Mouse Monoclonal Antibody [Clone ID: 9F9.F9]
Specifications
| Product Data | |
| Clone Name | 9F9.F9 |
|---|---|
| Application | ELISA, FC, IF, IHC, WB |
| Recommended Dilution | WB: 1:3,000 - 1:30,000 IHC: 1:1,000 - 1:5,000 IF: User Optimized FC: User Optimized ELISA: 1:10,000 - 1:30,000 |
| Reactivity | eGFP, GFP, rGFP |
| Antibody Host | Mouse |
| Isotype | IgG1, kappa |
| Clonality | Monoclonal |
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. |
| Specificity | GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Protein A affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Database Link | |
| Background | Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. |
| Synonyms | mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish |
| Note | Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. |
| Reference Data | |
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Citations
Protein kinase C gamma regulates Purkinje cell dendritic spine development in a mouse model of spinocerebellar ataxia
Sziber, Torrents-Solé, Kovacevic et al
Exp Neurol (2025) 393, 115377 DOI: 10.1016/j.expneurol.2025.115377
Sziber, Torrents-Solé, Kovacevic et al
Exp Neurol (2025) 393, 115377 DOI: 10.1016/j.expneurol.2025.115377
Cited by 4
Multiple roles of single-minded 2 in esophageal squamous cell carcinoma and its clinical implications
Tamaoki, Komatsuzaki, Komatsu et al
Cancer Sci. (2018) 109 (4), 1121-1134 DOI: 10.1111/cas.13531
Tamaoki, Komatsuzaki, Komatsu et al
Cancer Sci. (2018) 109 (4), 1121-1134 DOI: 10.1111/cas.13531
AI Summary: OriGene products pCMV6-AC-GFP-SIM2, pCMV6-SIM2s, and pCMV6-neo were used to transfect cells with different SIM2 isoforms, enabling the study of SIM2's roles in tumor differentiation and chemoradiotherapy sensitivity in esophageal squamous cell carcinoma.
Snippet: pCMV6‐AC‐GFP containing the long isoform of the SIM2 cDNA and pCMV6‐neo containing SIM2s cDNA were purchased from OriGene Technologies (Rockville, MD, USA). Cells were plated at 2 × 106 per 10‐cm dish, and transfected with either pCMV6‐AC‐GFP‐SIM2 or pCMV6‐SIM2s or no insert of pCMV6‐neo (OriGene Technologies) by using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's protocol. … Colonies were transferred to larger plates, and expression of different splicing isoforms of SIM2 mRNAs was examined by quantitative real‐time PCR
Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs
Liang, Nichols, Sun et al
Nucleic Acids Res. (2017)
Liang, Nichols, Sun et al
Nucleic Acids Res. (2017)
Cited by 23
AI Summary: OriGene’s anti-GFP antibody (TA50041) was used for immunoprecipitation of GFP-tagged proteins from cell lysates to investigate the interactions between RNase H1-dependent antisense oligonucleotides and their mRNA targets in the study.
Snippet: …Since cytoplasmic mRNAs ar.;Cell lysates were prepared as described above for polysome profile. 350 μl cell lysate was added to Protein A/G magnetic beads pre-coated with 15 μg anti-GFP antibody (TA50041, Origene) or mouse IgG, incubated at 4°C for 2 h, and washed five times with wash buffer (20 mM Tris pH 7.5, 5 mM MgCl2, 150 mM KCl, 0.1% NP40, supplemented with RNaseOut)
Single-molecule studies on the label number distribution of fluorescent markers
Grußmayer, Kurz, Herten
Chemphyschem (2014) 15 (4), 734-42 DOI: 10.1002/cphc.201300840
Grußmayer, Kurz, Herten
Chemphyschem (2014) 15 (4), 734-42 DOI: 10.1002/cphc.201300840
Cited by 23
AI Summary: In this study, OriGene products—specifically, the SNAP-tag protein from OriGene—were used to investigate and compare the label number distribution of fluorescent markers at the single-molecule level, demonstrating that SNAP-tagged proteins typically incorporate only a single fluorescent label per molecule.
Showing 1-4 of 4 papers.
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