ycjM Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000 IP: 1:100 |
| Reactivity | Escherichia coli |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Sucrose Phosphorylase E.coli |
| Specificity | This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Sucrose Phosphorylase E.coli. Cross reactivity against Sucrose Phosphorylase from other sources is unknown. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL |
| Concentration | 90 mg/mL - lot specific |
| Purification | Prepared from monospecific antiserum by a delipidation and defibrination. |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Sucrose phosphorylase catalyzes as Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate. |
| Synonyms | goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase |
| Note | Anti-Sucrose phosphorylase has been tested by western blot and is suitable to be assayed against 1.0 ug of Sucrose Phosphorylase E.coli in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG L - Cat# H (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. |
| Reference Data | |
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