PKM (pan m-PK) Goat Polyclonal Antibody
$2,045.00
3 Days*
Specifications
| Product Data | |
| Application | ELISA, IHC, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:5,000 - 1:20,000 IHC: User Optimized ELISA: 1:50,000 - 1:200,000 IP: User Optimized |
| Reactivity | Rabbit |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Pyruvate Kinase Rabbit Muscle |
| Specificity | Anti-PYRUVATE KINASE Antibody immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Pyruvate Kinase Rabbit Muscle. This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL |
| Concentration | 40 mg/mL - lot specific |
| Purification | Prepared from monospecific antiserum by delipidation and defibrination. |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Pyruvate kinase (EC: 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a magnesium ion. The enzyme is a 'transferase' under the international classification of enzymes. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. However, in anaerobic glycolysis, lactate dehydrogenase will utilize the NADH produced by glyceraldehyde phosphate dehydrogenase to reduce pyruvate to lactate. In humans, there are two pyruvate kinase isozymes: type M (muscle, SwissProt P14618) and type L,R (liver and erythrocyte, SwissProt P30613). The isozymes differ in primary structure and regulation. This reaction has a large positive free energy change, one of three in glycolysis. All three such steps regulate the overall activity of the pathway, and are, in general, irreversible under wild-type conditions. Anti-Pyruvate kinase antibody is idea for investigators involved in cytokines and growth factor research. |
| Synonyms | goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2 |
| Note | Anti-PYRUVATE KINASE Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Specific conditions should be optimized by user. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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