HXK1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:3,000 IHC: User Optimized ELISA: 1:5,000 - 1:25,000 |
| Reactivity | Hexokinase (Yeast) |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Hexokinase Yeast |
| Specificity | This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Hexokinase Yeast. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL |
| Concentration | 80 mg/mL - lot specific |
| Purification | Prepared from monospecific antiserum by a delipidation and defibrination. |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Hexokinase is the main glucose phosphorylating enzyme. It may play a regulatory role in both induction and repression of gene expression by glucose. In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism. |
| Synonyms | rabbit anti-Hexokinase Antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody |
| Note | Anti-Hexokinase is suitable for use in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. |
| Reference Data | |
Reviews
Documents
| Product Manuals |
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| SDS |
Citations
Eliyahu, Pnueli, Melamed et al
Mol Cell Biol (2010) 30 (1), 284-94 DOI: 10.1128/MCB.00651-09
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