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GFP Goat Polyclonal Antibody

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SKU
R1091T
Anti-GFP (GOAT) Antibody Rhodamine Conjugated (Min X Hu Ms & Rt Serum Proteins), 1 mg
Size:
1 mg
Conjugation:
TRITC
  $1,130.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application FLISA, IF, WB
Recommended Dilution WB: >1:10,000
IF: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivity eGFP, GFP, rGFP
Antibody Host Goat
Clonality Polyclonal
Immunogen Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity GFP Rhodamine Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.  No reaction was observed against Human, Mouse and Rat Serum Proteins.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 1.0 mg/mL - lot specific
Purification Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Conjugation TRITC
Storage Store Anti GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Stability Expiration date is one (1) year from date of receipt.
Shipping Ambient
Database Link
Background Anti-GFP antibody is Rhodamine labeled. Conjugated GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms goat anti-GFP Antibody Rhodamine Conjugation, TRITC conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Note Goat Anti-GFP Rhodamine Conjugated Antibody has been tested by dot blot and western blot and is useful in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reference Data
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Documents
Resources

file-alt Citations

Aberrant neurogenesis after stroke: a retroviral cell labeling study
Niv, Keiner, Krishna et al
Stroke (2012) 43 (9), 2468-75 DOI: 10.1161/STROKEAHA.112.660977
AI Summary: In this study, OriGene’s anti-GFP antibody (Cat# TA150041) was used to detect and analyze GFP-labeled newborn neurons, enabling the assessment of their morphological integration into hippocampal circuitry after stroke.
Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells
Suzuki, Mogami, Ihara et al
Blood (2009) 113 (2), 470-8 DOI: 10.1182/blood-2008-03-144279
Cited by 47
AI Summary: In this study, OriGene’s tPA-GFP construct (RC217571) and PAI-1 siRNA (SR304112) were used to visualize and manipulate tissue plasminogen activator and PAI-1 expression in vascular endothelial cells, enabling detailed analysis of their secretory dynamics and interactions.
Showing 1-2 of 2 papers.
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