GOT1 Sheep Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:3,000 ELISA: 1:5,000 - 1:25,000 |
| Reactivity | Pig |
| Antibody Host | Sheep |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Aspartate Aminotransferase (AST) Pig Heart |
| Specificity | Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) Pig Heart. Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Anti-Aspartate Transaminase antibody is present in the cytoplasm of cells, belonging to the class-I pyridoxal-phosphate-dependent aminotransferase family. Playing an active role in amino acid metabolism, Anti-Aspartate Transaminase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. |
| Synonyms | sheep anti-Aspartate Transaminase biotin conjugated Antibody, biotin conjugated sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody |
| Note | Anti-Aspartate Transaminase has been assayed against 1.0 ug of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Reference Data | |
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