Folate receptor alpha Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:1,000 ELISA: 1:1,000 - 1:2,500 IP: 1:100 |
| Reactivity | Bovine |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Folate Binding Protein Bovine Milk |
| Specificity | Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Folate Binding Protein Bovine Milk. Cross reactivity against Folate Binding Protein from other sources is unknown. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Anti-Folate Binding Protein antibody belongs to the folate receptor family and binds to folate and reduced folic acid derivatives. It further regulates delivery of 5-methyltetrahydrofolate to the cell interior. Folate expresses solely in epithelial tissues, and expression is increased in malignant tissues. Anti-Folate Binding Protein antibody is ideal for researchers investigating Tags & Cell Markers and Cancer research. |
| Synonyms | goat anti-Folate Binding Protein Antibody biotin Conjugation, biotin Conjugated goat anti-Folate Binding Protein Antibody, Folate receptor alpha, FR-alpha, Folate receptor 1, Milk folate-binding protein, Folate-binding protein 1, FBP |
| Note | Anti-Folate Binding Protein has been tested by western blot and is suitable to be assayed against 1.0 ug of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Reference Data | |
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