Carbonic Anhydrase II (CA2) Rabbit Polyclonal Antibody
$840.00
3 Days*
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,500 ELISA: 1:2,000 - 1:10,000 |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Carbonic Anhydrase II Human Erythrocytes |
| Specificity | Anti-Carbonic Anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II Human Erythrocytes. Cross reactivity against Carbonic Anhydrase II from other sources may occur but has not been specifically determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Gene Name | carbonic anhydrase 2 |
| Database Link | |
| Background | Anti-Carbonic Anhydrase II antibody is necessary for bone resorption and osteoclast differentiation and is involved in the reversible hydration of carbon dioxide. It plays a role in the modulating of fluid secretion into the anterior chamber of the eye. Belonging to the alpha-carbonic anhydrase family, Anti-Carbonic Anhydrase II antibody is ideal for investigators involved in the areas of Cardiovascular and Cell Biology research. |
| Synonyms | rabbit anti-Carbonic Anhydrase II Antibody biotin Conjugation, biotin conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II |
| Note | Anti-Carbonic Anhydrase II Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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