lacZ Rabbit Polyclonal Antibody
$825.00
3 Days*
Specifications
| Product Data | |
| Application | IF, WB |
|---|---|
| Recommended Dilution | WB: User Optimized IF: User Optimized |
| Reactivity | b-GAL |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Beta Galactosidase (E. coli) |
| Specificity | Anti-Beta Galactosidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Beta Galactosidase (E. coli). |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
| Conjugation | FITC |
| Storage | Store vial antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research. |
| Synonyms | rabbit anti-Beta Galactosidase Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal FITC conjugated antibody, β-Gal Fluorescein, Anti-β-Gal Fluorescein Antibody |
| Note | Anti-Beta Galactosidase Fluorescein Conjugated Antibody has been tested by dot bot and western blot and is designed for fluorescent western blotting and immunofluorescence. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Reference Data | |
Reviews
Documents
| Product Manuals |
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Citations
Lymphocytes from P2X7-deficient mice exhibit enhanced P2X7 responses
Taylor, Gonzalez-Begne, Sojka et al
J Leukoc Biol (2009) 85 (6), 978-86 DOI: 10.1189/jlb.0408251
Taylor, Gonzalez-Begne, Sojka et al
J Leukoc Biol (2009) 85 (6), 978-86 DOI: 10.1189/jlb.0408251
Cited by 49
AI Summary: In this study, OriGene’s anti-P2X7 antibody (catalog #TA326177) was used to detect P2X7 protein expression, contributing to the characterization of P2X7 activity in lymphocytes from P2X7-deficient mice.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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