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ALDOA Goat Polyclonal Antibody

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SKU
R1054PS
Anti-Aldolase (Rabbit Muscle) (GOAT) Antibody, 100 ug
Size:
100 ug
Conjugation:
Unconjugated
  $870.00
3 Days*
Bulk/Customize

View other "ALDOA or NP_001075707.1" antibodies

Specifications
Specifications
Product Data
Application ELISA, IP, WB
Recommended Dilution WB: 1:1,000 - 1:5,000
ELISA: 1:7,000
IP: 1:100
Reactivity Human, Rabbit
Antibody Host Goat
Clonality Polyclonal
Immunogen Aldolase Rabbit Muscle
Specificity Anti-ALDOLASE was prepared from monospecific antiserum by a delipidation, salt fractionation and ion exchange chromatography.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase Rabbit Muscle.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration 1 mg/ml - lot specific
Purification Prepared from monospecific antiserum by a delipidation, slat fractionation and ion exchange chromatography
Conjugation Unconjugated
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Stability Expiration date is one (1) year from date of receipt.
Shipping Ambient
Database Link
Background Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, it may also function as scaffolding protein. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in the liver, and aldolase C in the brain. Alkylation of Arg-43 inactivates the enzyme. Aldolase is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Synonyms goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase
Note Anti-Aldolase Antibody has been tested by ELISA, immunoprecipitation, and western blot. This product is assayed against 1.0 µg of Aldolase Rabbit Muscle in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG L - Cat# H (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 is suggested for this product.  Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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