Apolipoprotein A II (APOA2) Goat Polyclonal Antibody

CAT#: R1031P

Apolipoprotein A II (APOA2) goat polyclonal antibody, Purified

Product Images

Apolipoprotein AII antibody staining of Paraffin-Embedded Human Adrenal at 5 ug/ml.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: This Apo AII antibody R1031P has been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and Western blotting for highly sensitive qualitative analysis.
  Recommended Dilutions:
  ELISA: 1/5,000-1/10,000.
  Immunoprecipitation: 1/100.
  Western blot: 1/5,000-1/10,000.
  Immunohistochemistry: 1/50-1/200.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: ApoLipoprotein Type A-II was isolated from Human plasma by Density Gradient Centrifugation followed by HPLC purification.
• Specificity: This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
  Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.
  This antibody reacts with ApoLipoprotein A-II and has negligible cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins.
  Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible.
• Formulation: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 and 0.01% Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (mix with an equal volume of glycerol and aliquot) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: apolipoprotein A2
• Database Link:
  Entrez Gene 336 Human
  UniProt ID P02652
• Background: Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.
• Synonyms: Apolipoprotein A-II, Apolipoprotein A2, Apo-AII, ApoA-II, APOA2