TAP1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:3,000 ELISA: 1:5,000 - 1:25,000 |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Transporter Associated Protein (TAP I ) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Specificity | This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) |
| Database Link | |
| Background | TAP1 (Transporter 1, ATP Binding Cassette Subfamily B Member) Members of the MDR/TAP subfamily are involved in multidrug resistance. TAP1 is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. It also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. It is inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, celiac disease, Bare Lymphocyte Syndrome, Type I and Immunodeficiency by Defective Expression Of Hla Class 1. Anti-TAP1 Antibody is ideal for researchers interested in the Innate Immune System and Class I MHC. |
| Synonyms | rabbit anti-TAP1 antibody, rabbit anti-ABCB2 antibody, TAP 1 antibody, PSF1 antibody, RING4 antibody, TAP1N antibody, Antigen peptide transporter 1, ATP-binding cassette sub-family B member 2, Transporter 1 ATP binding cassette sub family B (MDR/TAP) antibody |
| Note | This product was assayed by ELISA against TAP-I peptide in an antibody sandwich assay using Peroxidase conjugated Affinity Purified anti-Rabbit IgG L - Cat# H (Goat) code #611-1302. A dilution of 1:10,000 is suggested from this experiment. No reaction was observed against TAP-II. This product was assayed by immunoblotting and was reactive against the 72 kDa human TAP-I protein at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG L - Cat# H (Goat) code #611-1302. No reaction was observed against TAP-II. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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