Neutrophils Rat Monoclonal Antibody [Clone ID: 7/4]

Name: Neutrophils Rat Monoclonal Antibody [Clone ID: 7/4]
Brand: OEM
SKU: CL050LE
Price: 1255 USD
Availability: InStock

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SKU

CL050LE

Neutrophils rat monoclonal antibody, clone 7/4, Low Endotoxin

Datasheet SDS

<strong>Published customer image:</strong> <br>Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (<strong>MCA771GA</strong>) used for the detection of Ly-6B.2 positive cells by immunofluorescence.<br><strong>Image caption:</strong><br>CX3CR1+, Ly6Clo monocytes are present in perihematomal brain in CX3CR1-null BM chimeras. Mice were injected intracardially with 150μg tomato lectin to delineate vasculature. Brain slices were stained with Ly6B.2 antibody to differentiate Ly6Chi monocytes and neutrophils from Ly6Clo CX3CR1+ monocytes and Dapi nuclear stain. A) Intracardiac injection of lectin stains vasculature in the brain– 20x. B) At 72 hours after ICH, distinct subsets of amoeboid Ly6B.2+ (Ly6Chi monocytes or neutrophils) and GFP+ (CX3CR1+) monocytes are seen in the perihematomal region. 20x. C) At 7 days after ICH, amoeboid, Ly6B.2−,CX3CR1+ cells are seen in the brain parenchyma surrounding the ICH cavity. The center of the ICH cavity is filled with Ly6B.2+ (Ly6Chi) monocytes or neutrophils. Tomato lectin staining is also seen in the center of the ICH cavity, most likely due to blood brain barrier breakdown at this time point leading to nonspecific lectin staining of myeloid cells. 10x. White box indicates the inset shown in panel E. D) At 14 days, amoeboid, CX3CR1+ cells can still be seen in the perihematomal region, however more CX3CR1+ cells have a ramified morphology. No Ly6B.2+ (Ly6Chi monocytes or neutrophils) cells are found. Overall blood-brain barrier disruption is improved leading to less lectin staining in the cavity, although there remain some lectin+ cells (CX3CR1+ monocytes and CX3CR1− microglia). 10x. White box indicates inset shown in panel F. E) 20x image of panel C – perihematomal region 7 days after ICH F) 20x image of panel D – perihematomal region 14 days after ICH. Blue – Dapi, GFP – CX3CR1, Red – Tomato lectin, Pink – Ly6B.2, n = 5.<br><br>From: Taylor RA, Hammond MD, Ai Y, Sansing LH (2014)<br>CX3CR1 Signaling on Monocytes Is Dispensable after Intracerebral Hemorrhage.<br><strong><a href="https://www.ncbi.nlm.nih.gov/pubmed/25469644"target="_blank" rel="noopener">PLoS ONE 9(12): e114472</a></strong>.<br>This image is from an open access article distributed under the terms of a <a href="https://creativecommons.org/licenses/by/4.0/"target="_blank" rel="noopener">Creative Commons Attribution License</a>.

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Specifications

Specifications
Product Data
Clone Name	7/4
Application	FC, IHC, WB
Recommended Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen and Paraffin Sections. Western blot. Immunoprecipitation.
Reactivity	Mouse
Antibody Host	Rat
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Cultured bone marrow cells. Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
Specificity	This antibody recognises a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for clone 7/4: 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains. Negative/Weak for clone 7/4: A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6.
Buffer	PBS without preservatives. State: Low Endotoxin State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage	Store the antibody undiluted at -20°C only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability	Shelf life: one year from despatch.
Shipping	Blue Ice
Note	Endotoxin Level: < 0.01 EU/Ug
Reference Data

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Documents

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
SDS for CL050LE

Resources

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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Neutrophils Rat Monoclonal Antibody [Clone ID: 7/4]

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Neutrophils Rat Monoclonal Antibody [Clone ID: 7/4]

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