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Cd4 Rat Monoclonal Antibody [Clone ID: CT-CD4]

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SKU
CL004R
Cd4 rat monoclonal antibody, clone CT-CD4, PE
Size:
50 ug
Conjugation:
PE
  $580.00
2 Weeks*
Bulk/Customize

View other "Cd4 or NP_038516.1" antibodies

Specifications
Specifications
Product Data
Clone Name CT-CD4
Application FC
Recommended Dilution Flow Cytometry (See Protocols).
(Reported to be useful in Immunohistochemistry on Acetone Fixed Frozen Sections).
Reactivity Mouse
Antibody Host Rat
Isotype IgG2a
Clonality Monoclonal
Specificity This monoclonal antibody (mAb) recognizes Mouse CD4 (L3T4) which is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells.
Buffer PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: PE
State: Liquid purified IgG fraction.
Concentration lot specific
Conjugation PE
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This antibody is photosensitive and should be protected from light.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Gene Name CD4 antigen
Database Link
Background Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC's and in signal transduction via protein tyrosine kinase lck1.
Synonyms T-cell surface antigen T4/Leu-3
Note Protocol: FLOW CYTOMETRY ANALYSIS:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test).
4. To each tube, add ~1.0 µg of this Ab per 106 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + Sodium Azide (100 µl of 2M Sodium Azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine Serum Albumin + Sodium Azide (100 µl of 2M Sodium Azide in 100 mls).

Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x106 cells per test
Antibody Concentration Used: 1.0 µg/106 cells
Isotypic Control: PE Rat IgG2a
Reference Data
Protein Categories ADC Targets, CD Markers, Immune system diseases, Intracellular Proteins, Membrane Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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