PKR (EIF2AK2) Mouse Monoclonal Antibody [Clone ID: OTI3F5]

SKU CF813289

Carrier-free (BSA/glycerol-free) EIF2AK2 mouse monoclonal antibody,clone OTI3F5

Product Data

• Clone Name: OTI3F5
• Application: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 51-191 of human EIF2AK2 (NP_001129123) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 61.9 kDa
• Gene Name: eukaryotic translation initiation factor 2 alpha kinase 2
• Database Link:
  NP_001129123
  P19525
• Background: The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Synonyms: EIF2AK1; LEUDEN; PKR; PPP1R83; PRKR

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors