MCF2 Mouse Monoclonal Antibody [Clone ID: OTI2C12]

SKU CF812536

Carrier-free (BSA/glycerol-free) MCF2 mouse monoclonal antibody,clone OTI2C12

Product Data

• Clone Name: OTI2C12
• Application: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 872-1001 of human MCF2 (NP_001093325) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Gene Name: MCF.2 cell line derived transforming sequence
• Database Link:
  NP_001165347
  P10911
• Background: The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity. [provided by RefSeq, Jan 2010]
• Synonyms: ARHGEF21; DBL

Reference Data

• Protein Families: Druggable Genome