RIAM (APBB1IP) Mouse Monoclonal Antibody [Clone ID: OTI6E3]

CAT#: CF809230

Carrier-free (BSA/glycerol-free) APBB1IP mouse monoclonal antibody,clone OTI6E3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY APBB1IP (Cat# RC208712, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1IP (Cat# TA809230)(1:2000). Positive lysates LY412750 (100ug) and LC412750 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-APBB1IP monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-APBB1IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809230) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-APBB1IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809230) (1:500) (1:250)

Specifications

Product Data

• Clone Name: OTI6E3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:250
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human APBB1IP (NP_061916) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73 kDa
• Gene Name: amyloid beta precursor protein binding family B member 1 interacting protein
• Database Link:
  NP_061916
  Entrez Gene 54518 Human
  UniProt ID Q7Z5R6
• Synonyms: INAG1; PREL1; RARP1; RIAM