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CD40 Mouse Monoclonal Antibody [Clone ID: OTI1F12]

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SKU
CF807575
Carrier-free (BSA/glycerol-free) CD40 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "CD40 or NM_001250" antibodies

Specifications
Specifications
Product Data
Clone Name OTI1F12
Application IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 21-166 of human CD40(NP_001241) produced in E.coli.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 28.4 kDa
Gene Name CD40 molecule
Database Link
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul
Synonyms Bp50; CDW40; p50; TNFRSF5
Reference Data
Protein Categories ADC Targets, CD Markers, Immune system diseases, Membrane Proteins, Secreated Proteins
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways Allograft rejection, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Primary immunodeficiency, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Viral myocarditis
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Other "OTI1F12" antibodies (4)
SKU Description Size Price
TA807575 CD40 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12) 100 ul
$447.00
TA807575AM CD40 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12), Biotinylated 100 ul
$509.00
TA807575BM CD40 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12), HRP conjugated 100 ul
$509.00
TA807575S CD40 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12) 30 ul
$200.00

file-alt Citations

Molecular signature of slowly expanding lesions in progressive multiple sclerosis
Jäckle, Zeis, Schaeren-Wiemers et al
Brain (2020) 143 (7), 2073-2088 DOI: 10.1093/brain/awaa158
Cited by 103
AI Summary: OriGene products, specifically the Human ALOX15B (NM_001140) qPCR Primer Pair (HP208324) and Human MME (NM_000902) qPCR Primer Pair (HP210855), were used in this study to validate increased gene expression of ALOX15B and MME in slowly expanding lesions of progressive multiple sclerosis by quantitative PCR.
Snippet: Jackle et al. profile slowly expanding lesions in progressive multiple sclerosis, and reveal a preferential accumulation of M1-type differentiated microglia alo.;The following antibodies were used to identify microglia and M1- or M2-differentiated macrophages/microglia: anti-TMEM119 (Sigma-Aldrich) (Satoh et al., 2016) was used as microglia-specific marker;anti-CD40 (Acris Antibodies), anti-iNOS (Chemicon International Inc.) and anti-NOX1 (Sigma-Aldrich) were used as M1 markers;anti-CD206 (AbD SeroTec) and anti-CD163 (Laboratories Novocastra ) were used as M2 markers
IHC Array
Overexpression of CCN1 in Het1A cells attenuates bile-induced esophageal metaplasia through suppressing non-canonical NFκB activation
Dang, Meng, Modak et al
Cytokine (2019) 116, 61-69 DOI: 10.1016/j.cyto.2018.12.020
AI Summary: OriGene products contributed to this study by providing plasmids and antibodies (specifically NFKB1, NFKB2, CD40, and LTBR antibodies) used for cell staining and molecular analyses to investigate the mechanisms of NFκB activation in esophageal cells.
Snippet: All the plasmids were purchased from Origene, Rockville, USA The following antibodies were used in cell staining: MUC2, KRT4, KRT20, VIL1, and CDX2 (Abcam);NFKB1, NFKB2, CD40, and LTBR (Origene). 2.4. Real-Time RT-PCR analysis
ICC
Showing 1-2 of 2 papers.
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