BCL2 Mouse Monoclonal Antibody [Clone ID: OTI9D3]
SKU
CF806591
Carrier-free (glycerol/BSA-free) BCL2 mouse monoclonal antibody, clone OTI9D3 (formerly 9D3)
$620.00
3 Days*
Specifications
| Product Data | |
| Clone Name | OTI9D3 |
|---|---|
| Application | IHC, WB |
| Recommended Dilution | WB 1:2000, IHC 1:150 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human BCL2 (NP_000624) produced in HEK293T cell. |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 26.1 kDa |
| Gene Name | BCL2 apoptosis regulator |
| Database Link | |
| Synonyms | Bcl-2; PPP1R50 |
| Reference Data | |
| Protein Categories | Cancer: Gynecologic, Cancer: Haematopoietic and Lymphoid, Cancer: Head and Neck, Cancer: Lung, Cancer: Sarcoma, Cytokines, Growth Factors, Intracellular Proteins, Membrane Proteins |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI9D3" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA806591 | BCL2 mouse monoclonal antibody, clone OTI9D3 (formerly 9D3) | 100 ul |
$447.00
|
|
| TA806591AM | BCL2 mouse monoclonal antibody, clone OTI9D3 (formerly 9D3), Biotinylated | 100 ul |
$509.00
|
|
| TA806591BM | BCL2 mouse monoclonal antibody, clone OTI9D3 (formerly 9D3), HRP conjugated | 100 ul |
$509.00
|
|
| TA806591S | BCL2 mouse monoclonal antibody, clone OTI9D3 (formerly 9D3) | 30 ul |
$200.00
|
|
Citations
3-Bromopyruvic acid regulates glucose metabolism by targeting the c-Myc/TXNIP axis and induces mitochondria-mediated apoptosis in TNBC cells
Li, Pan, Liu et al
Exp Ther Med (2022) 24 (2), 520 DOI: 10.3892/etm.2022.11447
Li, Pan, Liu et al
Exp Ther Med (2022) 24 (2), 520 DOI: 10.3892/etm.2022.11447
Cited by 21
AI Summary: In this study, OriGene’s c-Myc antibody (TA802714) and TXNIP antibody (TA500494) were used for western blot analysis to assess the expression levels of key proteins involved in glucose metabolism and apoptosis in TNBC cells, helping demonstrate the molecular effects of 3-bromopyruvic acid treatment.
Impact of NF-κB pathway on the apoptosis-inflammation-autophagy crosstalk in human degenerative nucleus pulposus cells
Yi, Wen, Tan et al
Aging (Albany NY) (2019) 11 (17), 7294-7306 DOI: 10.18632/aging.102266
Yi, Wen, Tan et al
Aging (Albany NY) (2019) 11 (17), 7294-7306 DOI: 10.18632/aging.102266
AI Summary: In this study, OriGene’s Bcl-2 and Bax antibodies were used to assess apoptosis-related protein expression, helping to elucidate the role of the NF-κB pathway in regulating apoptosis, inflammation, and autophagy in human degenerative nucleus pulposus cells.
Snippet: the membranes were blocked with 5% nonfat dry milk. Sequentially, the membranes were incubated with primary anti-IL-1β, TNF-α (Santa Cruz, USA), Bcl-2, Bax (OriGene, Herford, Germany), P65, LC3B, P62, p-AKT, AKT, p-mTOR and mTOR (Cell Signaling Technology, USA), β-actin (Beyotime, China) overnight at 4 °C
Defective activation of the MAPK/ERK pathway, leading to PARP1 and DNMT1 dysregulation, is a common defect in IgA nephropathy and Henoch-Schönlein purpura
Milillo, Molinario, Costanzi et al
J. Nephrol. (2018)
Milillo, Molinario, Costanzi et al
J. Nephrol. (2018)
AI Summary: OriGene contributed to this study by providing the anti-BCL2 antibody, which was used to assess BCL2 protein expression levels as part of the molecular analysis of the MAPK/ERK pathway in IgA nephropathy and Henoch-Schönlein purpura patients.
Snippet: and quanti- fied with Image J. In this study, the following primary antibodies were used: anti-human IgA (Novus Biological, 1:5000), anti- human ERK1/2 (Cell Signalling 1:1000), anti-human phospho ERK1/2 (Pierce 1:1000, Cell Signalling 1:2000), anti-BCL2 (Origene
Showing 1-3 of 3 papers.
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