GAPDH Mouse Monoclonal Antibody [Clone ID: OTI2F8]
SKU
CF802563
Carrier-free (BSA/glycerol-free) GAPDH mouse monoclonal antibody, clone OTI2F8 (formerly 2F8)
$620.00
3 Days*
Specifications
| Product Data | |
| Clone Name | OTI2F8 |
|---|---|
| Application | IHC, WB |
| Recommended Dilution | WB 1:2000, IHC 1:150 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human GAPDH (NP_002037) produced in HEK293T cell. |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 35.9 kDa |
| Gene Name | glyceraldehyde-3-phosphate dehydrogenase |
| Database Link | |
| Background | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Feb 2012 |
| Synonyms | G3PD; GAPD; HEL-S-162eP |
| Reference Data | |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI2F8" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA802563 | GAPDH mouse monoclonal antibody, clone OTI2F8 (formerly 2F8) | 100 ul |
$447.00
|
|
| TA802563AM | GAPDH mouse monoclonal antibody,clone 2F8, Biotinylated | 100 ul |
$509.00
|
|
| TA802563BM | GAPDH mouse monoclonal antibody,clone 2F8, HRP conjugated | 100 ul |
$509.00
|
|
| TA802563S | GAPDH mouse monoclonal antibody, clone OTI2F8 (formerly 2F8) | 30 ul |
$200.00
|
|
Citations
Efficacy of Obcordata A from Aspidopterys obcordata on Kidney Stones by Inhibiting NOX4 Expression
Li, Ma, Lv et al
Molecules (2019) 24 (10) DOI: 10.3390/molecules24101957
Li, Ma, Lv et al
Molecules (2019) 24 (10) DOI: 10.3390/molecules24101957
AI Summary: OriGene products contributed to this study by providing antibodies against NOX4, P38, JNK, OPN, MCP-1, and GAPDH, which were used for immunoblotting and protein expression analysis.
Snippet: 4. Materials and Methods. 4.1. Antibodies and Reagents. The antibodies against NOX4, P38, JNK, OPN, MCP-1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from OriGene Technologies (Rockville, MD, USA) Cached;
Feasibility analysis of treating severe intrauterine adhesions by transplanting menstrual blood-derived stem cells
Zheng, Wang, Wang et al
Int. J. Mol. Med. (2018) 41 (4), 2201-2212 DOI: 10.3892/ijmm.2018.3415
Zheng, Wang, Wang et al
Int. J. Mol. Med. (2018) 41 (4), 2201-2212 DOI: 10.3892/ijmm.2018.3415
AI Summary: OriGene products, specifically the anti-vimentin antibody (catalog no. TA802563S), were used in this study to detect vimentin protein expression by western blot, helping to assess the differentiation of menstrual blood-derived stem cells into endometrial cells.
Snippet: no. TA802563S), all 1:100 dilution;OriGene Technologies, Inc.] overnight at 4˚C. Subsequent to being washed three times, the blots were incubated with the appropriate horseradish peroxidase‑conjugated secondary antibody (Cell Signaling Technology, Danvers
Activation of anaphase-promoting complex by p53 induces a state of dormancy in cancer cells against chemotherapeutic stress
Dai, Wang, Tang et al
Oncotarget (2016) 7 (18), 25478-92 DOI: 10.18632/oncotarget.8172
Dai, Wang, Tang et al
Oncotarget (2016) 7 (18), 25478-92 DOI: 10.18632/oncotarget.8172
Cited by 36
AI Summary: OriGene’s GAPDH antibody (TA802563) was used as a loading control in this study to ensure accurate measurement of protein expression levels during the investigation of cancer cell dormancy mechanisms.
Snippet: p21 (10355-1-AP, Proteintech,), cyclin A2 (18202-1-AP, Proteintech,), cyclin B1 (55004-1-AP, Proteintech), slug (12129-1-AP, Proteintech);smad2 (86F7, Cell Signaling Technology, Danvers, MA);Histone 3 (AH433, Beyotime Biotechnology, China);GAPDH (TA802563, Origene, USA)
Showing 1-3 of 3 papers.
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