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TLE 1 (TLE1) Mouse Monoclonal Antibody [Clone ID: OTI1D6]

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SKU
CF800301
Carrier-free (BSA/glycerol-free) TLE1 mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "TLE 1 or NM_005077" antibodies

Specifications
Specifications
Product Data
Clone Name OTI1D6
Application FC, IF, IHC
Recommended Dilution IHC 1:150
IF 1:100
FLOW 1:100
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 (NP_005068) produced in E.coli.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 83 kDa
Gene Name TLE family member 1, transcriptional corepressor
Database Link
Synonyms ESG; ESG1; GRG1
Reference Data
Protein Categories Intracellular Proteins
Protein Families Transcription Factors
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Other "OTI1D6" antibodies (5)
SKU Description Size Price
TA700270 TLE1 biotinylated mouse monoclonal detection antibody, validated for ELISA and Luminex assays 50 ug
$494.00
TA800301 TLE1 mouse monoclonal antibody, clone OTI1D6 (formerly 1D6) 100 ul
$447.00
TA800301AM TLE1 mouse monoclonal antibody,clone 1D6, Biotinylated 100 ul
$509.00
TA800301BM TLE1 mouse monoclonal antibody,clone 1D6, HRP conjugated 100 ul
$509.00
TA800301S TLE1 mouse monoclonal antibody, clone OTI1D6 (formerly 1D6) 30 ul
$200.00

file-alt Citations

Reduced expression of the co-regulator TLE1 in type 2 diabetes is associated with increased islet α-cell number
Armour, Anderson, Richardson et al
Endocrinology (2020)
Cited by 10
AI Summary: OriGene’s TLE1 mouse monoclonal antibody (TA800301) was used in this study to detect TLE1 protein expression in pancreatic islet cells, enabling the authors to assess its reduced levels and association with increased α-cell number in type 2 diabetes.
Snippet: Nat Genet. 2012;44(9): 981-990. 19. RRID:AB_2625368, https://www.origene.com/ catalog/antibodies/primary- antibodies/ta800301/tle-1-tle1-mouse-monoclonal-antibody-clone- id-oti1d6 20. RRID:AB_306130, https://www.abcam.com/insulin-antibody-ab7842 21
IF
HDAC and Proteasome Inhibitors Synergize to Activate Pro-Apoptotic Factors in Synovial Sarcoma
Laporte, Barrott, Yao et al
PLoS ONE (2017) 12 (1), e0169407 DOI: 10.1371/journal.pone.0169407
AI Summary: OriGene’s TLE1 antibody (TA800301) was used for immunodetection to study protein expression changes in synovial sarcoma cells treated with HDAC and proteasome inhibitors.
Snippet: Wells were blocked with blocking buffer and incubated overnight at 4°C with primary antibodies at a 1:1000 dilution: SS18 (Santa-Cruz Biotechnology, Dallas, TX, USA, sc-28698), TLE1 (Origene Technologies, Rockville, MD, USA, TA800301).
FigureFigureFigure
Identification of cytotoxic agents disrupting synovial sarcoma oncoprotein interactions by proximity ligation assay
Laporte, Ji, Ma et al
Oncotarget (2016)
Cited by 14
AI Summary: In this study, the OriGene TLE1 antibody (TA800301) was used for immunohistochemistry to investigate the interactions of the SS18-SSX oncoprotein in synovial sarcoma cells.
Snippet: Immunohistochemistry staining was carried out using the Ventana automated staining platform, with primary antibody at a 1/150 dilution for both SS18 (Santa- Cruz Biotechnologies, sc-28698) and TLE1 (Origene, TA800301). Proximity ligation assay ... Cite SaveSaving
IHC
Targeted activity of histone deacetylase inhibition in synovial sarcoma
Laporte
Thesis (2017)
Snippet: FFPE tissue and embedded cell line pellets were sectioned at 4 μm onto micro - slides for use in both conventional immunohistochemistry and proximity ligat ion assay procedures. Immunohistochemistry staining was carried out using the Ventana automated staining platform, with primary antibody at a 1/150 dilution for both SS18 (Santa - Cruz Biotechnologies , Dallas, TX, USA sc - 28698 ) and TLE1 (Origene , Rockville, MD, USA TA800301 )
IHC
Showing 1-4 of 4 papers.
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