MRPS27 Mouse Monoclonal Antibody [Clone ID: OTI6E5]

CAT#: CF800244

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Carrier-free (BSA/glycerol-free) MRPS27 mouse monoclonal antibody, clone OTI6E5 (formerly 6E5)

Formulation: Standard Carrier-Free



USD 600.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI6E5
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 47.4 kDa
Gene Name Homo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2, mRNA; nuclear gene for mitochondrial product.
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Synonyms MRP-S27; S27mt
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.