HSCB Mouse Monoclonal Antibody [Clone ID: OTI2B7]
SKU
CF507285
Carrier-free (glycerol/BSA-free) HSCB mouse monoclonal antibody, clone OTI2B7 (formerly 2B7)
$620.00
3 Days*
Specifications
| Product Data | |
| Clone Name | OTI2B7 |
|---|---|
| Application | WB |
| Recommended Dilution | WB 1:4000 |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human HSCB(NP_741999) produced in HEK293T cell |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 27.2 kDa |
| Gene Name | HscB mitochondrial iron-sulfur cluster cochaperone |
| Database Link | |
| Synonyms | DNAJC20; HSC20; JAC1 |
| Reference Data | |
| Protein Categories | Cardiovascular diseases, Intracellular Proteins |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI2B7" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA507285 | HSCB mouse monoclonal antibody, clone OTI2B7 (formerly 2B7) | 100 ul |
$447.00
|
|
| TA507285AM | HSCB mouse monoclonal antibody, clone OTI2B7 (formerly 2B7), Biotinylated | 100 ul |
$509.00
|
|
| TA507285BM | HSCB mouse monoclonal antibody, clone OTI2B7 (formerly 2B7), HRP conjugated | 100 ul |
$509.00
|
|
| TA507285S | HSCB mouse monoclonal antibody, clone OTI2B7 (formerly 2B7) | 30 ul |
$200.00
|
|
Citations
Heme biosynthesis depends on previously unrecognized acquisition of iron-sulfur cofactors in human amino-levulinic acid dehydratase
Liu, Sil, Maio et al
Nat Commun (2020) 11 (1), 6310 DOI: 10.1038/s41467-020-20145-9
Liu, Sil, Maio et al
Nat Commun (2020) 11 (1), 6310 DOI: 10.1038/s41467-020-20145-9
Cited by 78
AI Summary: In this study, the pCMV6-Entry vector from OriGene was used as an empty vector control in immunoprecipitation experiments to assess the role of iron-sulfur clusters in human ALAD function during heme biosynthesis.
Snippet: h no EDTA (Roche)]. The background, corresponding to 55Fe measurements of eluates after anti-FLAG immunoprecipitations on cytosolic extracts from cells transfected with the empty vector (pCMV6-Entry;Origene), was subtracted from each reading. Overexpression and anaerobic purification of maltose-binding protein (MBP)-tagged human ALAD proteins BL21(DE3) competent bacterial cells (NEB) were cotran
Showing 1-1 of 1 papers.
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