IDO1 Mouse Monoclonal Antibody [Clone ID: OTI2G4]
SKU
CF506367
Carrier-free (BSA/glycerol-free) IDO1 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)
$620.00
3 Days*
Specifications
| Product Data | |
| Clone Name | OTI2G4 |
|---|---|
| Application | IF, IHC, WB |
| Recommended Dilution | WB 1:200~2000, IHC 1:150, IF 1:100 |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human IDO1(NP_002155) produced in HEk293T cell. |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 45.1 kDa |
| Gene Name | indoleamine 2,3-dioxygenase 1 |
| Database Link | |
| Background | This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. provided by RefSeq, Feb 2011 |
| Synonyms | IDO; IDO-1; INDO |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Tryptophan metabolism |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI2G4" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA506367 | IDO1 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4) | 100 ul |
$447.00
|
|
| TA506367AM | IDO1 mouse monoclonal antibody,clone 2G4, Biotinylated | 100 ul |
$509.00
|
|
| TA506367BM | IDO1 mouse monoclonal antibody,clone 2G4, HRP conjugated | 100 ul |
$509.00
|
|
| TA506367S | IDO1 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4) | 30 ul |
$200.00
|
|
Citations
Differential kynurenine pathway metabolism in highly metastatic aggressive breast cancer subtypes: beyond IDO1-induced immunosuppression
Heng, Bilgin, Lovejoy et al
Breast Cancer Res (2020) 22 (1), 113 DOI: 10.1186/s13058-020-01351-1
Heng, Bilgin, Lovejoy et al
Breast Cancer Res (2020) 22 (1), 113 DOI: 10.1186/s13058-020-01351-1
Cited by 58
AI Summary: In this study, OriGene products—including the KMO and KYNU antibodies—were used for western blot and immunohistochemistry analyses to assess protein expression of key kynurenine pathway enzymes in breast cancer subtypes, enabling the identification of their upregulation in aggressive cancers.
Influence of Indoleamine-2,3-Dioxygenase and Its Metabolite Kynurenine on γδ T Cell Cytotoxicity against Ductal Pancreatic Adenocarcinoma Cells
Jonescheit, Oberg, Gonnermann et al
Cells (2020) 9 (5) DOI: 10.3390/cells9051140
Jonescheit, Oberg, Gonnermann et al
Cells (2020) 9 (5) DOI: 10.3390/cells9051140
Cited by 40
AI Summary: OriGene products, specifically the anti-IDO-1 monoclonal antibody (clone 1A3) and anti-IDO-2 monoclonal antibody (clone 1A4), were used to detect and analyze IDO protein expression in pancreatic ductal adenocarcinoma cells by Western blotting in this study.
Snippet: In total, 106 PDAC cells were cultured in medium or treated with 100 ng/mL or 500 ng/mL rIFN-γ (R&D Systems) for 48 h. Thereafter, cells were lysed in TNE lysis buffer with 1% (v/v) Nonidet P-40 (Fluka Chemie, Buchs, Switzerland) in 50 mM Tris (Roth, Karlsruhe, Germany), 2 mM EDTA, 150 mM NaCl (Merck, Darmstadt, Karlsruhe), with phosphatase and protease inhibitors sodium fluoride and sodium orthov...
Radiological Features of IDO1+/PDL1+ Lung Adenocarcinoma: A Retrospective Single-institution Study
Takada, Toyokawa, Tagawa et al
Anticancer Res. (2018) 38 (9), 5295-5303 DOI: 10.21873/anticanres.12856
Takada, Toyokawa, Tagawa et al
Anticancer Res. (2018) 38 (9), 5295-5303 DOI: 10.21873/anticanres.12856
AI Summary: In this study, OriGene’s mouse monoclonal anti-IDO1 antibody (clone UMAB126) was used for immunohistochemistry to assess IDO1 expression in lung adenocarcinoma tissue samples.
Snippet: IHC analysis was conducted using commercially available antibodies against IDO1 at 1:200 dilution (mouse monoclonal, clone UMAB126;Origene Technologies, Rockville, MD, USA), and PDL1 at 1:100 dilution (rabbit monoclonal, clone SP142;Spring Bioscience
Showing 1-3 of 3 papers.
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