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Her2 (ERBB2) Mouse Monoclonal Antibody [Clone ID: OTI4F10]

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SKU
CF503443
Carrier-free (BSA/glycerol-free) ERBB2 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "Her2 or NM_004448" antibodies

Specifications
Specifications
Product Data
Clone Name OTI4F10
Application FC, IF, IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 137.7 kDa
Gene Name erb-b2 receptor tyrosine kinase 2
Database Link
Background This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. provided by RefSeq
Synonyms CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Note Antibody specificy evaluation by using OriGene Protein microarray chip and WB analysis showed that this antibody cross-reacts with CDH1 recombinant protein in overexpression lysates.
Reference Data
Protein Categories ADC Targets, Cancer: Bladder, Cancer: Brain and CNS, Cancer: Gastrointestinal, Cancer: Gynecologic, Cancer: Head and Neck, CAR-T Targets, CD Markers, Enzyme: Kinases, Growth Factors, Intracellular Proteins, Membrane Proteins
Protein Families Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer
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Other "OTI4F10" antibodies (6)
SKU Description Size Price
TA503443 ERBB2 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10) 100 ul
$447.00
TA503443AM ERBB2 mouse monoclonal antibody,clone 4F10, Biotinylated 100 ul
$509.00
TA503443BM ERBB2 mouse monoclonal antibody,clone 4F10, HRP conjugated 100 ul
$509.00
TA503443S ERBB2 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10) 30 ul
$200.00
TA600208 ERBB2 mouse monoclonal capture antibody, validated for Luminex assays 100 ug
$458.00
TA700213 ERBB2 biotinylated mouse monoclonal detection antibody, validated for Luminex assays 50 ug
$494.00

file-alt Citations

The role of the size of affinity ligands in the detection and characterization of extracellular vesicles
Pham, Chowdhury, Patel et al
Biosens Bioelectron (2024) 258, 116381 DOI: 10.1016/j.bios.2024.116381
Cited by 10
AI Summary: OriGene's anti-human HER2 HRP-conjugated antibody (Cat No.: TA503443BM) was used as a comparator to evaluate the detection sensitivity of Affibody molecules versus traditional antibodies in identifying HER2-positive extracellular vesicles.
Snippet: anti-human CD63 biotin-conjugated antibody (BioLegend, Cat No.: 353017), anti-human CD63 FITC-conjugated antibody (BioLegend, Cat No.: 353006), anti-human HER2 HRP-conjugated antibody (OriGene, Cat No.: TA503443BM), anti-human HER2 FITC-conjugated antibody (BioLegend, Cat No.: 324404), anti-human HER2 FITC-conjugated antibody (Thermo Fisher Scientific, Cat No.: BMS120FI)
ELISA
Metaplastic carcinoma of the breast: Imaging and pathological features
Bian, Lin, Wu et al
Oncol Lett (2016) 12 (5), 3975-3980 DOI: 10.3892/ol.2016.5177
Cited by 33
AI Summary: OriGene products, specifically the anti-ER (estrogen receptor), anti-PR (progesterone receptor), anti-CerbB-2, and anti-P53 antibodies, were used for immunohistochemical staining to assess the expression of these markers in breast tissue samples, helping characterize the pathological features of metaplastic carcinoma in this study.
Decreased sirtuin 4 expression is associated with poor prognosis in patients with invasive breast cancer
Shi, Liu, Zhang et al
Oncol Lett (2016) 12 (4), 2606-2612 DOI: 10.3892/ol.2016.5021
Cited by 52
AI Summary: OriGene products contributed to this study by providing the anti-p53 (catalog no. TA502780) and anti-Ki-67 (catalog no. TA500265) antibodies used for immunohistochemical analysis of breast cancer tissue samples.
Snippet: TA502780;dilution, 1:100;incubation time, 15 min;tempera- ture, 25˚C) and Ki‑67 (catalog no. TA500265;dilution, 1:50;incubation time, 20 min;temperature, 25˚C) antibodies (Origene Technologies Inc., Rockville, MD, USA). ... Cite SaveSaving
IHC
Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation
Choi, de Poot, Lee et al
Nat Commun (2016) 7, 10963 DOI: 10.1038/ncomms10963
Cited by 108
AI Summary: In this study, OriGene's anti-ERbB2 antibody (TA503443) was used to detect ERbB2 protein levels, contributing to the analysis of proteasome substrate degradation in cells expressing open-gate proteasome mutants.
Snippet: Sources of antibodies and working dilutions are as follows: anti-tau (clone Tau-5;Invitrogen, USA, 1/10,000);anti-tauser396 (ab109390, Abcam, USA, 1/5,000);anti-tauser199/202 (ab4864, Abcam, 1/5,000);anti-β-actin (A1978, Sigma, 1/10,000);anti-Ub (clone P4D1, Santa Cruz, USA, 1/5,000), anti-Ub conjugates (clone FK2, Enzo Life Science, USA, 1/2,500), anti-Ub (Lys48 specific) (clone apu2, Millipore, USA, 1/2,000), anti-Ub (Lys63 specific) (clone apu3, Millipore, 1/2,000), anti-α3 (PW8115, Enzo Life Science, 1/5,000), anti-α7 (PW8110, Enzo Life Sciences, 1/3,000), anti-ADRM1 (PW9910, Enzo Life Science, 1/2,000), anti-His (A03001, IgTherapy, Korea, 1/2,000), anti-T7 (69522, Millipore, 1/5,000), anti-flag (F1804, Sigma, 1/3,000), anti-Rpt5 (PW8245, Enzo Life Science, 1/3,000), anti-NBR1 (sc130380, Santa Cruz, 1/2,000), anti-p62 (sc28359, Santa Cruz, 1/2,000), anti-p53 (sc1313, Santa Cruz, 1/3,000), anti-LC3 (L7543, Sigma, 1/2,000), anti-USP14 (A300-920A, Bethyl Laboratories, USA, 1/2,000), anti-GFP antibody (Enogene, USA, 1/5,000), anti-LTB4 (bs-5779R, Bioss USA, 1/1,000), anti-FKBP3 (A302-601A, Bethyl, 1/1,000), anti-FKBP4 (A301-426A, Bethyl, 1/1,000), anti-TOR3A (AP17612c, Abgent, 1/1,000), anti-Calnexin (A303-694A, Bethyl, 1/1,000), anti-EPHB2 (bs-0996R, Bioss USA, 1/1,000), anti-ERbB2 (TA503443, OriGene, 1/1,000), anti-ITGB1 (A303-735A, Bethyl, 1/1,000), anti-UNC5B (EB11706, Everest, 1/1,000), anti-TOP2B (C0376, Assay Biotech, 1/1,000), anti-SGPL1 (bs-4188R, Bioss, USA, 1/1,000), anti-DCDC2 (bs-11824R, Bioss, USA, 1/1,000), anti-ITGA4 (4783, ProSci, 1/1,000), anti-ApoB (bs-6333R, Bioss, USA, 1/1,000), anti-SCARB1 (5193, ProSci, 1/1,000), and anti-VAPB (A302-894A, Bethyl, 1/1,000. …
FigureFigureFigure
WB
Showing 1-4 of 4 papers.
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