XPF (ERCC4) Mouse Monoclonal Antibody [Clone ID: OTI4E11]

SKU CF503261

Carrier-free (BSA/glycerol-free) ERCC4 mouse monoclonal antibody, clone OTI4E11 (formerly 4E11)

Product Data

• Clone Name: OTI4E11
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERCC4(NP_005227) produced in HEK293 cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 104.3 kDa
• Gene Name: ERCC excision repair 4, endonuclease catalytic subunit
• Database Link:
  NP_005227
  Q92889
• Background: The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).
• Synonyms: ERCC11; FANCQ; RAD1; XFEPS; XPF

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair