Apg3 (ATG3) Mouse Monoclonal Antibody [Clone ID: OTI2C12]

SKU CF503161

Carrier-free (BSA/glycerol-free) ATG3 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)

Product Data

• Clone Name: OTI2C12
• Application: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:50~100, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATG3(NP_057393) produced in HEK293 cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 35.7 kDa
• Gene Name: autophagy related 3
• Database Link:
  NP_071933
  Q9NT62
• Background: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM]
• Synonyms: APG3; APG3-LIKE; APG3L; PC3-96

Reference Data

• Protein Pathways: Regulation of autophagy