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LCMT1 Mouse Monoclonal Antibody [Clone ID: OTI1G5]

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SKU
CF503127
Carrier-free (BSA/glycerol-free) LCMT1 mouse monoclonal antibody, clone OTI 1G5 (formerly 1G5)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "LCMT1 or NM_016309" antibodies

Specifications
Specifications
Product Data
Clone Name OTI1G5
Application FC, IF, IHC
Recommended Dilution IHC 1:150
IF 1:100
FLOW 1:100
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human LCMT1(NP_057393) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 38.2 kDa
Gene Name leucine carboxyl methyltransferase 1
Database Link
Background LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 PubMed 10600115). supplied by OMIM, Mar 2008
Synonyms CGI-68; LCMT; PPMT1
Reference Data
Protein Categories Intracellular Proteins
Protein Pathways Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
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Other "OTI1G5" antibodies (5)
SKU Description Size Price
TA503127 LCMT1 mouse monoclonal antibody, clone OTI 1G5 (formerly 1G5) 100 ul
$447.00
TA503127AM LCMT1 mouse monoclonal antibody, clone OTI 1G5 (formerly 1G5), Biotinylated 100 ul
$509.00
TA503127BM LCMT1 mouse monoclonal antibody, clone OTI 1G5 (formerly 1G5), HRP conjugated 100 ul
$509.00
TA503127S LCMT1 mouse monoclonal antibody, clone OTI 1G5 (formerly 1G5) 30 ul
$200.00
TA700109 LCMT1 biotinylated mouse monoclonal detection antibody, validated for Luminex assays 50 ug
$494.00

file-alt Citations

Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1
Tang, Lu, Mo et al
Life Sci. (2018)
AI Summary: OriGene contributed anti-PME-1 (TA500823) and LCMT1 (TA503127) antibodies used for detecting and analyzing the expression and localization of PME-1 and LCMT1 proteins in this study.
Snippet: The indicated antibodies were incubated with the membranes. Protein bands were detected by ECL and quantified by ImageJ. Anti-PME-1 and LCMT1 antibodies were obtained from Origene (TA500823, TA503127, Origene, Rockville, MD)
WB IF
H2 O2 induces PP2A demethylation to downregulate mTORC1 signaling in HEK293 cells
Tang, Qin, Wang et al
Cell Biol. Int. (2018)
AI Summary: OriGene contributed to this study by providing the anti-LCMT1 antibody (TA503127), which was used to detect LCMT1 protein levels in experiments investigating the regulation of mTORC1 signaling by PP2A methylation under oxidative stress.
Snippet: and protein bands were detected by ECL and quantified using ImageJ. Anti-LCMT1 antibody was obtained from Origene (TA503127, Origene, Rockville, MD). Anti-PP2Ac antibodies (unmethylated-PP2Ac, methylated-PP2Ac, total PP2Ac) were purchased from Abcam
Showing 1-2 of 2 papers.
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