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PDHA1 Mouse Monoclonal Antibody [Clone ID: OTI2B10]

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SKU
CF502704
Carrier-free (BSA/glycerol-free) PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
4 Weeks*
Bulk/Customize

View other "PDHA1 or NM_000284" antibodies

Specifications
Specifications
Product Data
Clone Name OTI2B10
Application FC, IF, WB
Recommended Dilution WB 1:2000, IF 1:100, FLOW 1:100
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human PDHA1 (NP_000275) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 40.2 kDa
Gene Name pyruvate dehydrogenase E1 subunit alpha 1
Database Link
Background The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Synonyms PDHA; PDHAD; PDHCE1A; PHE1A
Reference Data
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
Protein Pathways Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, leucine and isoleucine biosynthesis, Metabolic pathways, Pyruvate metabolism, Valine
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Other "OTI2B10" antibodies (4)
SKU Description Size Price
TA502704 PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) 100 ul
$447.00
TA502704AM PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10), Biotinylated 100 ul
$509.00
TA502704BM PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10), HRP conjugated 100 ul
$509.00
TA502704S PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) 30 ul
$200.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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