DEF8 Mouse Monoclonal Antibody [Clone ID: OTI4B6]

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SKU
CF502669
Carrier-free (BSA/glycerol-free) DEF8 mouse monoclonal antibody, clone OTI4B6 (formerly 4B6)
  $620.00
4 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name OTI4B6
Application FC, IF, IHC, WB
Recommended Dilution WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Full length human recombinant protein of human DEF8 (NP_997397) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 58.5 kDa
Gene Name differentially expressed in FDCP 8 homolog
Database Link
Synonyms FLJ20186; MGC104349
Reference Data
Protein Categories Intracellular Proteins
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Other "OTI4B6" antibodies (4)
SKU Description Size Price
TA502669 DEF8 mouse monoclonal antibody, clone OTI4B6 (formerly 4B6) 100 ul
$447.00
TA502669AM DEF8 mouse monoclonal antibody, clone OTI4B6 (formerly 4B6), Biotinylated 100 ul
$509.00
TA502669BM DEF8 mouse monoclonal antibody, clone OTI4B6 (formerly 4B6), HRP conjugated 100 ul
$509.00
TA502669S DEF8 mouse monoclonal antibody, clone OTI4B6 (formerly 4B6) 30 ul
$200.00

file-alt Citations

Involvement of autophagic protein DEF8 in Lewy bodies
Tanaka, Miki, Bettencourt et al
Biochemical and Biophysical Research Communications (2022) 623, 170-175 DOI: 10.1016/j.bbrc.2022.07.069
Cited by 3
AI Summary: OriGene’s anti-DEF8 antibody (TA502669) was used in immunoblotting and immunohistochemical analyses to detect and quantify DEF8 protein levels, helping to demonstrate DEF8’s involvement in Lewy body formation in synucleinopathies.
Snippet: … Immunoblotting was performed as described previously [18]. Anti-DEF8 (TA502669;OriGene, Rockville, MD, USA;1:500) and anti-β actin (A5044;Sigma–Aldrich, St. …
WB IHC
Showing 1-1 of 1 papers.
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