Destrin (DSTN) Mouse Monoclonal Antibody [Clone ID: OTI2F7]

CAT#: CF502608

Carrier-free (BSA/glycerol-free) DSTN mouse monoclonal antibody, clone OTI2F7 (formerly 2F7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DSTN (Cat# RC203419, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DSTN(Cat# TA502608). Positive lysates LY402051 (100ug) and LC402051 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DSTN monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DSTN monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502608)

• Anti-DSTN mouse monoclonal antibody (TA502608) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DSTN (RC203419).

• HEK293T cells transfected with either RC203419 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DSTN antibody (TA502608), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-DSTN antibody (TA502608), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-DSTN antibody (TA502608), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2F7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DSTN (NP_006861) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.3 kDa
• Gene Name: Homo sapiens destrin, actin depolymerizing factor (DSTN), transcript variant 1, mRNA.
• Database Link:
  NP_006861
  Entrez Gene 477147 DogEntrez Gene 695969 MonkeyEntrez Gene 11034 Human
  UniProt ID P60981
• Background: The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: ACTDP; ADF; bA462D18.2; HEL32