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Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI3F11]

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SKU
CF501875
Carrier-free (BSA/glycerol-free) RNH1 mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "Ribonuclease Inhibitor or NM_002939" antibodies

Specifications
Specifications
Product Data
Clone Name OTI3F11
Application FC, IHC, Simple Western, WB
Recommended Dilution WB 1:500~2000, FLOW 1:100, IHC: 1:150; Simple Western 1:20-1:50
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 49.8 kDa
Gene Name ribonuclease/angiogenin inhibitor 1
Database Link
Background Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 PubMed 3219362). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo. supplied by OMIM
Synonyms RAI; RNH
Reference Data
Protein Categories Intracellular Proteins
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Other "OTI3F11" antibodies (4)
SKU Description Size Price
TA501875 RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 100 ul
$447.00
TA501875AM RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11), Biotinylated 100 ul
$509.00
TA501875BM RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11), HRP conjugated 100 ul
$509.00
TA501875S RNH1 (Ribonuclease Inhibitor) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) 30 ul
$200.00

file-alt Citations

Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR
Wang, Teferedegne, Shatzkes et al
Anal. Biochem. (2016)
Cited by 14
AI Summary: In this study, the OriGene monoclonal antibody against mammalian RNase inhibitor (clone 3F11; TA501875) was used to investigate the role of endogenous RNase inhibitor in maintaining RNA stability in crude cell lysates for RT-qPCR applications.
Snippet: involving lysates, the following additions were used: RNasin Plus (Promega;final concentration of 1 unit/μL), a monoclonal antibody (Ab) specific to mammalian RI (reactive across mammalian species including dog and human;clone 3F11;TA501875;Origene;1 μg ... Cite SaveSaving
Data on the inhibition of RNase inhibitor activity by a monoclonal antibody as assessed by microfluidics-based RNA electrophoresis
Wang, Teferedegne, Shatzkes et al
Data Brief (2016) 9, 417-421 DOI: 10.1016/j.dib.2016.09.010
Cited by 1
Snippet: was purified using the RNeasy kit (Qiagen) and diluted in Cell-Lysis (CL) Buffer (10 mM Tris–HCl pH 7.4, 0.25% Igepal CA-630, 150 mM NaCl) [2]. RNA (5 µg in 200 µL of CL Buffer) was mixed with 1 µg (1 µL) of monoclonal Ab specific to either RI (Origene;TA501875 ... Cited by 1 Cite SaveSaving
Showing 1-2 of 2 papers.
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