MIOX Mouse Monoclonal Antibody [Clone ID: OTI4G7]

CAT#: CF501421

Carrier-free (BSA/glycerol-free) MIOX mouse monoclonal antibody, clone OTI4G7 (formerly 4G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MIOX (Cat# RC210070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIOX(Cat# TA501421). Positive lysates LY413679 (100ug) and LC413679 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MIOX monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MIOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501421)

• Anti-MIOX mouse monoclonal antibody (TA501421) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MIOX (RC210070).

• HEK293T cells transfected with either RC210070 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIOX antibody (TA501421), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4G7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MIOX (NP_060054) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.8 kDa
• Gene Name: Homo sapiens myo-inositol oxygenase (MIOX), mRNA.
• Database Link:
  NP_060054
  Entrez Gene 55586 Human
  UniProt ID Q9UGB7
• Synonyms: ALDRL6

Reference Data

• Protein Pathways: Ascorbate and aldarate metabolism, Inositol phosphate metabolism