NIT2 Mouse Monoclonal Antibody [Clone ID: OTI2B9]
SKU
CF501109
Carrier-free (BSA/glycerol-free) NIT2 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)
$620.00
4 Weeks*
Specifications
| Product Data | |
| Clone Name | OTI2B9 |
|---|---|
| Application | FC, IF, IHC, WB |
| Recommended Dilution | WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell. |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 30.4 kDa |
| Gene Name | nitrilase family member 2 |
| Database Link | |
| Background | Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle |
| Synonyms | HEL-S-8a |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI2B9" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA501109 | Anti-NIT2 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9) | 100 ul |
$447.00
|
|
| TA501109AM | Anti-NIT2 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9), Biotinylated | 100 ul |
$509.00
|
|
| TA501109BM | Anti-NIT2 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9), HRP conjugated | 100 ul |
$509.00
|
|
| TA501109S | Anti-NIT2 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9) | 30 ul |
$200.00
|
|
Citations
ω-Amidase: an underappreciated, but important enzyme in L-glutamine and L-asparagine metabolism; relevance to sulfur and nitrogen metabolism, tumor biology and hyperammonemic diseases
Cooper, Shurubor, Dorai et al
Amino Acids (2015)
Cooper, Shurubor, Dorai et al
Amino Acids (2015)
Cited by 60
AI Summary: OriGene’s mouse monoclonal anti-hNit2/ω-amidase antibody was used for immunohistochemistry to localize ω-amidase in human tissues, supporting the study’s investigation of this enzyme’s metabolic roles.
Snippet: … Our preliminary evidence has provided support for this hypothesis. Specifically, we have used immunohistochemistry (hNit2/ω-amidase;OriGene;mouse monoclonal) and human hKAT I/GTK (Santa Cruz, goat polyclonal) on sections of human tissues using the Vector ABC immunoperoxidase kit [with 3,3′-diaminobenzidine (DAB) staining] (Fig. 4)
Showing 1-1 of 1 papers.
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