IDH3A Mouse Monoclonal Antibody [Clone ID: OTI2E9]
Specifications
| Product Data | |
| Clone Name | OTI2E9 |
|---|---|
| Application | FC, IF |
| Recommended Dilution | IF 1:100, Flow 1:100 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell. |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 39.6 kDa |
| Gene Name | isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha |
| Database Link | |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. |
| Synonyms | H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase 3 (NAD+) a; isocitrate dehydrogenase NAD subunit alpha; isocitric dehydrogenase; mitochondrial; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH |
| Reference Data | |
| Protein Categories | Intracellular Proteins, Nervous system Diseases |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI2E9" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA500912 | Anti-IDH3A mouse monoclonal antibody, clone OTI2E9 (formerly 2E9) | 100 ul |
$447.00
|
|
| TA500912AM | Anti-IDH3A mouse monoclonal antibody, clone OTI2E9 (formerly 2E9), Biotinylated | 100 ul |
$509.00
|
|
| TA500912BM | Anti-IDH3A mouse monoclonal antibody, clone OTI2E9 (formerly 2E9), HRP conjugated | 100 ul |
$509.00
|
|
| TA500912S | Anti-IDH3A mouse monoclonal antibody, clone OTI2E9 (formerly 2E9) | 30 ul |
$200.00
|
|
Citations
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