FH Mouse Monoclonal Antibody [Clone ID: OTI2A2]
SKU
CF500681
Carrier-free (BSA/glycerol-free) FH mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)
$620.00
3 Days*
Specifications
| Product Data | |
| Clone Name | OTI2A2 |
|---|---|
| Application | IF, WB |
| Recommended Dilution | WB 1:2000, IF 1:50~100 |
| Reactivity | Dog, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell. |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 50.2 kDa |
| Gene Name | fumarate hydratase |
| Database Link | |
| Background | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. provided by RefSeq |
| Synonyms | FMRD; HLRCC; LRCC; MCL; MCUL1 |
| Reference Data | |
| Protein Categories | Cancer: Bladder, Cancer: Endocrine, Endocrine and metabolic diseases, Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI2A2" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA500681 | FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI2A2 (formerly 2A2) | 100 ul |
$447.00
|
|
| TA500681AM | FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI2A2 (formerly 2A2), Biotinylated | 100 ul |
$509.00
|
|
| TA500681BM | FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI2A2 (formerly 2A2), HRP conjugated | 100 ul |
$509.00
|
|
| TA500681S | FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI2A2 (formerly 2A2) | 30 ul |
$200.00
|
|
Citations
HIRA loss transforms FH-deficient cells
Valcarcel-Jimenez, Rogerson, Yong et al
Sci Adv (2022) 8 (42), eabq8297 DOI: 10.1126/sciadv.abq8297
Valcarcel-Jimenez, Rogerson, Yong et al
Sci Adv (2022) 8 (42), eabq8297 DOI: 10.1126/sciadv.abq8297
Cited by 15
AI Summary: In this study, OriGene products such as the FH (OriGene #RC201307) and HIRA (OriGene #SR311083) shRNA plasmids were used to manipulate gene expression, enabling the researchers to investigate how HIRA loss affects the proliferation and transformation of FH-deficient cells.
Snippet: Fumarate hydratase (FH) is a mitochondrial enzyme that catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (TCA) cycle. Germline mutations of FH lead to hereditary leiomyomatosis and renal cell carcinoma (HLRCC), a cancer syndrome characterized by a highly aggressive form of renal cancer. Although HLRCC tumors metastasize rapidly, FH-deficient mice develop premalignant cysts in the kidneys, rather than carcinomas. …
Fumarate hydratase loss promotes mitotic entry in the presence of DNA damage after ionising radiation
Johnson, Costa, Ferguson et al
Cell Death Dis (2018) 9 (9), 913 DOI: 10.1038/s41419-018-0912-3
Johnson, Costa, Ferguson et al
Cell Death Dis (2018) 9 (9), 913 DOI: 10.1038/s41419-018-0912-3
AI Summary: OriGene’s FH Clone 2A2 mouse antibody (TA500681) was used in this study to detect fumarate hydratase protein levels, contributing to the analysis of FH loss and its effects on DNA damage response.
Snippet: The primary/secondary antibodies and their respective dilutions were as follows: V5 mouse 1:2000 Life Technologies R960-25. FH Clone 2A2 mouse 1:500 Origene TA500681. Calnexin Rabbit 1:1000 Abcam Ab22595. Phospho
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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