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Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: OTI6D11]

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SKU
CF500583
Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone OTI6D11 (formerly 6D11)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "Alpha B Crystallin or NM_001885" antibodies

Specifications
Specifications
Product Data
Clone Name OTI6D11
Application FC, IF, IHC, IP, Simple Western, WB
Recommended Dilution WB 1:500~1000, IHC 1:50, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Simple Western 1:100
Reactivity Human, Monkey, Mouse, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 20 kDa
Gene Name crystallin alpha B
Database Link
Background Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. provided by RefSeq
Synonyms CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2
Reference Data
Protein Categories Cardiovascular diseases, Intracellular Proteins, Membrane Proteins, Nervous system Diseases
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Other "OTI6D11" antibodies (4)
SKU Description Size Price
TA500583 CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11) 100 ul
$447.00
TA500583AM CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11), Biotinylated 100 ul
$509.00
TA500583BM CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11), HRP conjugated 100 ul
$509.00
TA500583S CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11) 30 ul
$200.00

file-alt Citations

A Multimodal characterization of olligodendrocyte-lineage cells and myelin in the postmortem human brain: Investigating the impact of childhood maltreatment
Perlman
Thesis (2026)
AI Summary: OriGene's Mouse anti-CRYAB antibody (cat# TA500583) was used in this study to label and analyze CRYAB protein expression in oligodendrocyte-lineage cells within postmortem human brain tissue.
Snippet: BSA blocking buffer (with normal donkey serum - NDS), Goat anti-Sox10 (R&D Systems, cat# AF2864, 1:100), Mouse anti-CRYAB (Origene, cat# TA500583, 1:100), and RNAseInhibitor (1:500) at 4 °C on a rotor
Characterizing Oligodendrocyte-Lineage Cells and Myelination in the Basolateral Amygdala: Insights from a Novel Methodology in Postmortem Human Brain
Perlman, Curto, Barnett-Burns et al
bioRxiv (2025)
Cited by 1
AI Summary: OriGene’s Mouse anti-CRYAB antibody (cat# TA500583) was used to immunolabel nuclei for the identification and sorting of oligodendrocyte-lineage cells in postmortem human brain tissue in this study.
Snippet: clei suspension was incubated for 1 h in 100 µl of 5% BSA blocking buffer (with normal donkey serum -NDS), Goat anti-Sox10 (R&D Systems, cat# AF2864, 1:100), Mouse anti-CRYAB (Origene, cat# TA500583, 1:100), and RNAseInhibitor (1:500) at 4°C on a rotor. The nuclei were then spun at 500g for 3 min at 4°C. …
FC IF FISH
Showing 1-2 of 2 papers.
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