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CDK5 Mouse Monoclonal Antibody [Clone ID: OTI2H6]

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SKU
CF500397
Carrier-free (BSA/glycerol-free) CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "CDK5 or NM_004935" antibodies

Specifications
Specifications
Product Data
Clone Name OTI2H6
Application FC, IF, WB
Recommended Dilution WB 1:1000~2000, IF 1:50~100, FLOW 1:100
Reactivity Dog, Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human CDK5 (NP_004926) produced in HEK293T cell.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 33.3 kDa
Gene Name cyclin dependent kinase 5
Database Link
Background Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
Synonyms PSSALRE
Reference Data
Protein Categories Enzyme: Kinases, Intracellular Proteins
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Alzheimer's disease, Axon guidance
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Other "OTI2H6" antibodies (4)
SKU Description Size Price
TA500397 CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) 100 ul
$447.00
TA500397AM CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6), Biotinylated 100 ul
$509.00
TA500397BM CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6), HRP conjugated 100 ul
$509.00
TA500397S CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) 30 ul
$200.00

file-alt Citations

Cyclin-dependent kinase 5 (Cdk5) activity is modulated by light and gates rapid phase shifts of the circadian clock
Brenna, Borsa, Saro et al
bioRxiv (2024)
Snippet: anti-Cdk5 clone 2H6 Mouse Origene CF500397 1:100 anti-GFP Rabbit Abcam ab6556 1:500 Anti-Creb Rabbit Cell signaling D76D11 1:200 Anti-Creb (pSer133) Rabbit Abcam Ab32096
Cyclin-dependent kinase 5 (CDK5) regulates the circadian clock
Brenna, Olejniczak, Chavan et al
Elife (2019) 8 DOI: 10.7554/eLife.50925
AI Summary: In this study, the OriGene mouse anti-Cdk5 antibody (clone 2H6) was used to detect CDK5 protein in tissue sections, enabling the authors to investigate CDK5’s role in regulating the mammalian circadian clock.
Snippet: fetal bovine serum (Gibco)/0.1% Triton X-100/1x TBS at RT. After the blocking, the primary antibodies, rabbit anti-PER2-1 1:200 (Alpha Diagnostic, Lot numb. 869900A1.2-L), mouse anti-Cdk5 clone 2H6 1:20 (Origene, Lot numb. …
FigureFigureFigure
IHC
Cyclin Dependent Kinase 5 (CDK5) Regulates the Circadian Clock
Brenna, Olejniczak, Chavan et al
bioRxiv (2019)
Snippet: After the blocking, the primary antibodies, rabbit 536 anti-PER2-1 1:200 (Alpha Diagnostic, Lot numb. 869900A1.2-L), mouse anti-Cdk5 clone 2H6 537 1:20 (Origene, Lot numb. A001), and rabbit anti-GFP 1:500 (abcam ab6556) diluted in 1%
Showing 1-3 of 3 papers.
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