Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: OTI1E1]

SKU CF500015

Carrier-free (BSA/glycerol-free) CK18 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)

Product Data

• Clone Name: OTI1E1
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, IF 1:100
• Reactivity: Human, Monkey, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 47.9 kDa
• Gene Name: keratin 18
• Database Link:
  NP_954657
  P05783
• Background: Keratin 18 together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
• Synonyms: CK-18; CYK18; K18

Reference Data

• Protein Pathways: Pathogenic Escherichia coli infection