PKM1 Mouse Monoclonal Antibody [Clone ID: OTI4E8]
Specifications
| Product Data | |
| Clone Name | OTI4E8 |
|---|---|
| Application | IHC, WB |
| Recommended Dilution | WB 1:500, IHC 1:150 |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide corresponding to the region encoded by PKM gene M1 isoform |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 57.9 kDa |
| Note | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. provided by RefSeq, May 2011. |
| Reference Data | |
Reviews
Documents
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Resources
Other "OTI4E8" antibodies (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA190162 | PKM1 mouse monoclonal antibody, clone OTI4E8 (formerly 4E8) | 100 ul |
$447.00
|
|
Citations
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