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IDH1 (R132H) mutant Rat Monoclonal Antibody [Clone ID: OTI3E12]

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SKU
CF190113
Carrier-free (BSA/glycerol-free) IDH1 mutant (R132H) rat monoclonal antibody, clone OTI3E12 (formerly 3E12)
Size:
100 ug
Formulation:
Carrier-free Standard
Conjugation:
Unconjugated
  $620.00
3 Days*
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View other "IDH1 (R132H) mutant" antibodies

Specifications
Specifications
Product Data
Clone Name OTI3E12
Application WB
Recommended Dilution WB 1:500
Reactivity Human
Antibody Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Synthetic peptide around the R132H mutation region of the human IDH conjugated to KLH
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Reference Data
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Other "OTI3E12" antibodies (4)
SKU Description Size Price
TA190113 IDH1 mutant (R132H) rat monoclonal antibody, clone OTI3E12 (formerly 3E12) 100 ul
$447.00
CF180016 Carrier-free (BSA/glycerol-free) Mouse monoclonal E2-Crimson antibody, Clone OTI3E12 100 ug
$620.00
TA180016 Mouse monoclonal E2-Crimson antibody, Clone OTI3E12 100 ul
$447.00
TA180016S Mouse monoclonal E2-Crimson antibody, Clone OTI3E12 30 ul
$200.00

file-alt Citations

Autopalmitoylation of IDH1-R132H regulates its neomorphic activity in cancer cells
Hu, Lin, Sun et al
Nat Chem Biol (2026)
Cited by 1
AI Summary: OriGene’s IDH1-R132H antibody (TA190113) was used in this study to specifically detect the mutant IDH1 protein, enabling the authors to investigate its autopalmitoylation and regulatory role in cancer cell metabolism.
Snippet: histone H3 (1:1,000;Santa Cruz, sc-517576), IDH1-R132H (1:1,000;OriGene, TA190113), anti-rabbit HRP (1:5,000;Cell Signaling, 7074S), anti-mouse HRP (1:5,000;Cell Signaling, 7076S) and anti-rat HRP (1:3,000;Cell Signaling, 7077S)
Enhancing Peptide and PMO Delivery to Mouse Airway Epithelia by Chemical Conjugation with the Amphiphilic Peptide S10
Auger, Sorroza-Martinez, Brahiti et al
Molecular Therapy - Nucleic Acids (2024)
Cited by 2
AI Summary: OriGene products, specifically the AAVS1 targeting vectors (CAT#: GE100023 and customized GE100024), were used to introduce an EGFP reporter cassette and selection marker into cells, enabling the generation and validation of modified cell lines for the study.
Snippet: resistant gene, an EGFP544 654 reporter cassette, and a homology arms to the AAVS1 locus sequence (OriGene 545 Technologies, CAT#: GE100023 and customized GE100024). Cells were selected for 546 puromycin resistance and single clones were isolated, validated, and used for in
Tumour microenvironment programming by an RNA-RNA-binding protein complex creates a druggable vulnerability in IDH-wild-type glioblastoma
Wu, Zhao, Shin et al
Nat Cell Biol (2024) 26 (6), 1003-1018 DOI: 10.1038/s41556-024-01428-5
Cited by 11
AI Summary: OriGene contributed to this study by providing the anti-mutant IDH1 antibody (TA190113), which was used to detect mutant IDH1 protein as part of the experimental analysis of glioblastoma samples.
Snippet: anti-HSP90α/β (F-8) (1:2,000 dilution, RRID:AB_675659;Santa Cruz Biotechnology, sc-13119), anti-Wip1 (1:1,000 dilution, RRID:AB_10986000;Santa Cruz Biotechnology, sc-376257) and anti-mutant IDH1 (1:2,000 dilution, RRID:AB_3076158;Origene, TA190113)
FigureFigureFigure
WB
Simultaneous targeting of DNA polymerase theta and PARP1 or RAD52 triggers dual synthetic lethality in homologous recombination-deficient leukemia cells
Sullivan-Reed, Toma, Drzewiecka et al
Mol Cancer Res (2023)
AI Summary: OriGene's IDH1(R132H) antibody (CF190113) was used in this study to detect the presence of mutant IDH1 protein, contributing to the characterization of leukemia cell models in experiments assessing the effects of DNA repair pathway inhibition.
Snippet: DNA polymerase theta (Poltheta, encoded by POLQ gene) plays an essential role in Poltheta-mediated end-joining (TMEJ) of DNA double-strand breaks (DSBs). Inhibition Save;PARP1 (Santa Cruz Biotech sc-74470), IDH1(R132H) (OriGene CF190113), histone H3 (Invitrogen, AHO1432), actin (Santa Cruz Biotech sc-47778) and Flag (Cell Signaling #2368)
WB
Low pH Facilitates Heterodimerization of Mutant Isocitrate Dehydrogenase IDH1-R132H and Promotes Production of 2-Hydroxyglutarate
Sesanto, Kuehn, Barber et al
Biochemistry (2021) 60 (25), 1983-1994 DOI: 10.1021/acs.biochem.1c00059
Cited by 10
AI Summary: OriGene’s anti-IDH1-R132H antibody (TA190113) was used to specifically detect the mutant IDH1-R132H protein in experiments analyzing dimer formation and 2-hydroxyglutarate production in this study.
Snippet: Plates were washed with wash buffer (binding buffer with 0.1% Tween 20) for 3 × 5 min and then incubated for 90 min with anti-IDH1 (rabbit, Cell Signaling 3997S, at 1:1000) for detecting WT:WT homodimer) or anti-IDH1-R132H (rat, Origene S TA190113, at 1:1000
ELISA
R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis
Qing, Dong, Gao et al
Mol Cell (2021) 81 (5), 922-939.e9 DOI: 10.1016/j.molcel.2020.12.026
Cited by 360
AI Summary: OriGene’s IDH1R132H (TA190113) product was used in this study to investigate the role of mutant IDH1 in leukemia cell metabolism and its impact on the FTO/m6A/PFKP/LDHB axis.
Snippet: LDHA (2012S, Cell Signaling Technology),FLAG M2 (F1804, Sigma-Aldrich), YTHDF2 (24744-1-AP, Proteintech), MYC (13978S, Cell Signaling Technology), IDH1R132H(TA190113, Origene)
Showing 1-6 of 6 papers.
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