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Hepatitis B Surface Antigen / HBsAg (ad/ay) Rabbit Polyclonal Antibody

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SKU
BP2029P
Hepatitis B Surface Antigen / HBsAg (ad/ay) rabbit polyclonal antibody, Purified
Size:
1 ml
Conjugation:
Unconjugated
  $1,190.00
2 Weeks*
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Specifications
Specifications
Product Data
Application ELISA
Recommended Dilution Suitable for use in ELISA. Also suitable for conjugation purposes.
Reactivity Hepatitis B Virus
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad & ay.
Specificity Reacts only with Hepatitis B surface antigen. This antibody is non-reactive with normal human serum.
Buffer 0.01M PBS, pH 7.2, with 0.09% sodium azide as preservative.
State: Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Protein A affinity chromatography (>95% pure).
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
Synonyms HBV surface antigen, Hepatitis B Virus
Reference Data
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Documents
Resources

file-alt Citations

Affinity-purified sHBsAg-based virus-like particles as a platform for foreign mRNA binding
Gackowska, Krejmer-Rabalska, Drazkowska et al
Virology (2025) 611, 110651 DOI: 10.1016/j.virol.2025.110651
Cited by 1
Snippet: After overnight wet transfer, the membrane was blocked with 5 % skimmed milk in TBS-T, and immunodetection was carried out using primary rabbit anti-HBsAg antibodies (1:1000, cat no. BP2029P, OriGene Technologies, USA) and secondary anti-rabbit alpaca antibodies conjugated with HRP (1:10000, Jackson ImmunoResearch Europe Ltd., UK)
WB
Minicircle-based vaccine induces potent T-cell and antibody responses against hepatitis C virus
Czarnota, Raszplewicz, Sławińska et al
Sci Rep (2024) 14 (1), 26698 DOI: 10.1038/s41598-024-78049-3
Cited by 6
AI Summary: OriGene’s anti-HBsAg antibody (cat no. BP2029P) was used in this study to detect HBV surface antigen expression, contributing to the evaluation of vaccine immunogenicity.
Snippet: were blocked overnight at 4 °C with 3% nonfat milk in TBST (TBS buffer, 0.1% (v/v) Tween-20). Following blocking, membranes were incubated for 1 h at RT with primary anti-HBsAg (dilution 1:1,000) (Origene;cat no. BP2029P), anti-NS3 (dilution 1:500) and anti-NS5 (dilution 1:200) (Santa Cruz Biotechnology;cat no. sc-69938 and sc-58146 respectively) antibodies diluted in nonfat milk in TBST, was
FigureFigureFigure
WB
Specific Antibodies Induced by Immunization with Hepatitis B Virus-Like Particles Carrying Hepatitis C Virus Envelope Glycoprotein 2 Epitopes Show Differential Neutralization Efficiency
Czarnota, Offersgaard, Pihl et al
Vaccines (Basel) (2020) 8 (2) DOI: 10.3390/vaccines8020294
Cited by 24
AI Summary: OriGene products, specifically the Myc-DDK-tagged HCV E1E2 plasmid (Cat# RC222327), were used in this study to express native E1E2 glycoprotein complexes for ELISA assays, enabling the evaluation of antibody reactivity induced by the hepatitis B virus-like particles carrying HCV E2 epitopes.
FigureFigureFigure
Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen
Lünsdorf, Gurramkonda, Adnan et al
Microb Cell Fact (2011) 10, 48 DOI: 10.1186/1475-2859-10-48
Cited by 94
AI Summary: In this study, OriGene’s anti-HBsAg monoclonal antibody (Cat# MBS666209) was used for immunocytochemical detection of hepatitis B surface antigen, enabling the visualization and localization of HBsAg within yeast cells during virus-like particle production.
FigureFigureFigure
Showing 1-4 of 4 papers.
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