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Mucin-Like Carcinoma Antigen (MCA) Mouse Monoclonal Antibody [Clone ID: b-12]

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SKU
BM4077
Mucin-Like Carcinoma Antigen (MCA) mouse monoclonal antibody, clone b-12, Aff - Purified
Size:
50 ug
Conjugation:
Unconjugated
  $1,195.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name b-12
Application IHC
Recommended Dilution Immunohistochemistry on:
Frozen sections: 0.5 µg/ml (1/200).
Paraffin sections: 4 µg/ml (1/25); Proteinase K pretreatment for antigen retrieval recommended.
Suggested positive control: Human uterus.
Antigen Distribution: In contrast to MCA producing tumors, the b-12 related antigen is only located at the MCA producing sites such as glandular cell surfaces or glandular tubuli. In MCA producing tumors, where cells become disorganized, the b-12 antigen is secreted into stromal tissue and blood vessels. (See Table 1.)
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Breast carcinoma cell lines.
Specificity b-12 is useful for identifying mucin-like carcinoma antigen (MCA) produced by various tumors and certain healthy glandular cells. In combination with other BMA markers for inflammation staging or investigating neo-vascularization processes b-12 is a valuable tool for studying tumor growth or regression. MCA is a 350 kDa glycoprotein with the typical biochemical characteristics of mucin-like glycoproteins (sialomucins) which protect surfaces. Antibody b-12 binds to the protein backbone of MCA, not to the large number of carbohydrate side chains. This antibody reacts with MCA producing cells.
MCA consists of a polymorphic family of glycoproteins. The b-12 related antigenic epitope is located in the more constant region of MCA.
Specificity for Human MCA producing cells.
Buffer PBS, buffer pH 7.2 with 0.05% Sodium Azide as preservative and 2 mg/ml BSA as stabilizer
State: Aff - Purified
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore with 0.5 ml distilled water.
Concentration 0.1 mg/ml
Purification Affinity Chromatography.
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Synonyms Marker for Mucin Producing Cells
Note Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum.

Protocol with formalin-fixed, paraffin-embedded sections:
The whole procedure is performed at room temperature
1. Deparaffinize and rehydrate tissue section
2. Incubate the tissue section with proteinase K for 7 min.
3. Wash in distilled water
4. Block endogenous peroxidase
5. Wash in PBS
6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
10. Wash in PBS
11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
12. Wash in PBS
13. Counterstain with Mayer's hemalum.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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