CD163 Mouse Monoclonal Antibody [Clone ID: ED2]
Specifications
| Product Data | |
| Clone Name | ED2 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow Cytometry: Use 10 µl of neat-1/10 diluted antiborde to label 106 cells in 100 µl. |
| Reactivity | Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line. |
| Specificity | This antibody recognizes the CD163 cell surface glycoprotein, a 175 kD molecule also known as ED2. Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti Rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). |
| Buffer | PBS, pH 7.2, containing 0.09% Sodium Azide and 1% BSA Label: FITC State: Liquid purified IgG fraction Label: Fluorescein Isothiocyanate Isomer 1 |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein A |
| Conjugation | FITC |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD163 molecule |
| Database Link | |
| Background | CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells. |
| Synonyms | M130, Hemoglobin scavenger receptor, Macrophage marker |
| Reference Data | |
| Protein Categories | ADC Targets, CD Markers, Intracellular Proteins, Membrane Proteins, Secreated Proteins |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
