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Desmoglein 2 (DSG2) Mouse Monoclonal Antibody [Clone ID: DG3.10]

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SKU
BM370
Desmoglein 2 (DSG2) mouse monoclonal antibody, clone DG3.10, Purified
Size:
50 ug
Conjugation:
Unconjugated
  $565.00
2 Weeks*
Bulk/Customize

View other "Desmoglein 2 or NP_001934" antibodies

Specifications
Specifications
Product Data
Clone Name DG3.10
Application IF, IHC, WB
Recommended Dilution Western blotting.
Immunofluorescence Microscopy.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Embedded Tissue (after microwave treatment).
Working Dilution: Dilute at least 1/10 with PBS, pH 7.4, for Immunohistochemical application.
Incubation time: 1 h at RT, extended with paraffin.
Reactivity Bovine, Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen "Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis
Specificity This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome-bearing cells. It does not react with cells containing plaque-bearing junctions of non-desmosomal type.
Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and meningiomas.
Polypeptide Reacting: Mr 165000 Desmoglein, "band 3" polypeptide of the desmosomal complex.
Epitope Recognized: Localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.
Tested Reactivities on Cultured Cell Lines: A-431, MCF-7, TR-146, PLC and various other Human epithelial cell lines; Bovine BMGE+H, BMGE-H, MDBK.
Buffer Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 1.0 ml distilled water.
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Gene Name desmoglein 2
Database Link
Background Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 and 2 are calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.
Synonyms DSG1, DSG-1, CDHF4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen, Desmoglein1, DSG2, DSG-2, CDHF5, HDGC, Desmoglein2
Reference Data
Protein Categories Cardiovascular diseases, Intracellular Proteins, Membrane Proteins
Protein Families Transmembrane
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC)
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Documents
Resources

file-alt Citations

Interleukin-10 Enhances the Intestinal Epithelial Barrier in the Presence of Corticosteroids through p38 MAPK Activity in Caco-2 Monolayers: A Possible Mechanism for Steroid Responsiveness in Ulcerative Colitis
Lorén, Cabré, Ojanguren et al
PLoS One (2015) 10 (6), e0130921 DOI: 10.1371/journal.pone.0130921
Cited by 56
AI Summary: In this study, OriGene’s anti-Desmoglein-2 antibody (Cat# TA809328) was used to assess desmoglein-2 expression in Caco-2 cell monolayers, contributing to the investigation of how IL-10 and glucocorticoids synergistically enhance intestinal epithelial barrier integrity.
FigureFigureFigure
In vitro functional analyses of arrhythmogenic right ventricular cardiomyopathy-associated desmoglein-2-missense variations
Gaertner, Klauke, Stork et al
PLoS One (2012) 7 (10), e47097 DOI: 10.1371/journal.pone.0047097
Cited by 20
AI Summary: OriGene products, specifically the DSG2 cDNA clone (RC217845) and the anti-DSG2 antibody (TA324705), were used in this study to express and detect wild-type and variant DSG2 proteins, enabling functional analyses of ARVC-associated DSG2 missense mutations.
FigureFigureFigure
Showing 1-2 of 2 papers.
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