PLEKHA4 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53347PU-N

PLEKHA4 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PLEKHA4 Antibody (N-term) in K562 cell line lysates (35ug/lane). This demonstrates the PLEKHA4 antibody detected the PLEKHA4 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with PLEKHA4 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PLEKHA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of K562 cells using PLEKHA4 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 150-1/100.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 28-58 amino acids from the N-terminal region of human PLEKHA4
• Specificity: This antibody recognizes Human PLEKHA4 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: pleckstrin homology domain containing A4
• Database Link:
  Entrez Gene 57664 Human
  UniProt ID Q9H4M7
• Background: PLEKHA4 binds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides.
• Synonyms: PEPP1, PEPP-1
• Note: Molecular Weight: 85401 Da